IF Cardio Cell Culture Dots

Segment cultured cardiac cells, detect cardiomyocytes and fibroblasts, and quantify dot markers (CISH/FISH) per cell,
IF Cardio Cell Culture Dots

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Overview

The IF Cardio Cell Culture Dot App provides cell segmentation, detection of cardiomyocytes (based an appropriate stain e.g. Troponin Red) and fibroblasts within cultured cardio cells plus one dot marker (CISH, FISH). The App outputs parameters such as number of cardiomyocytes, fibroblasts and dotpos cardiomyocytes, fibroblasts as well as number, area (μm²) and mean intensity of dots per cell.

Image courtesy of Agatha Ribeiro da Silva, Prof. Jose E. Krieger (Heart Institute, University Soa Paulo)

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